TSRL provides in-depth preclinical absorption and pharmacokinetic study support to help clients understand the absorption mechanism and the rate limiting steps to oral bioavailability. We specialize in several in vitro and in situ permeability models and also offer pharmacokinetic studies in rodents.

Discovery screening studies can be performed with either the in situ perfusion or in Caco-2 studies.  At TSRL, we place greater confidence in permeability determinations from the rat in situ perfusion model as it has clearly demonstrated superior correlation to human permeability data compared to tissue culture models. The in situ perfusion studies follow the same protocol as the BCS biowaiver (permeability) studies described below, but are conducted under non-GLP conditions. The in situ perfusion can be modified to answer specific project questions, i.e. transport mechanism, Pgp-mediated secretion and/or site specific permeability in different sections of the gastrointestinal tract.

• Preliminary BCS Permeability Classification in the Rat In Situ Perfusion Model
• GLP BCS Permeability Classification in the Rat In Situ Perfusion Model
• Transporter (hPept-1) over expressing cell lines (coming soon)
• Permeability Screening in the MDCK Cell Tissue Culture Model
• Mechanistic Permeability Studies in the Caco-2 Cell Tissue Culture Model
• Permeability Screening in the Caco-2 Cell Tissue Culture Model
• Permeability with mesenteric and/or systemic sampling (coming soon)
• Preclinical Pharmacokinetic Study in Rats
• Preclinical Pharmacokinetic Study in Mice